20X SSPE Buffer, pH 7.4, 8 X 500mL" Specification Value Concentration 20X Name SSPE Buffer pH at 1x pH 7. 4 Volume 8 X 500mL SKU SSP 20 0400 Composition at 1x NaCl 3. 0 M, NaH2PO4 0. 2 M, EDTA 0. 02 M A hybridization and wash buffer commonly used in Southern blotting, Northern blotting, and nucleic acid hybridization assays. This formulation provides optimal ionic strength for stable nucleic acid duplex formation and efficient probe binding. The buffer allows precise stringency