20X TBST Buffer, pH 7.6, 1L" Specification Value Concentration 20X Name TBST Buffer pH at 1x 7. 6 0. 2 Volume 1L SKU TBT 20 0100 Composition at 1x Tris 25mM, NaCl 137mM, KCl 2. 7mM, Tween 20 0. 05% A Tris buffered saline solution with Tween 20 detergent, serving as a standard washing and blocking buffer in western blotting, ELISA, and immunoassays. The Tween 20 component effectively reduces background signal by minimizing non specific protein binding to membranes and plate